Journal: Journal of pharmacological and toxicological methods

Article Title: Simulated Traumatic Brain Injury in In-Vitro Mouse Neuronal and Brain Endothelial Cell Culture Models

doi: 10.1016/j.vascn.2022.107159

Figure Lengend Snippet: List of Used Material

Article Snippet: Mouse Neuronal Cell Culture Celprogen 11003-02 One primary brain cell line we used in the protocol Mouse Neuronal Cell Culture Complete Growth Glucose and Serum Free Media Celprogen M11003-02GF Flushed with hypoxic gas for hypoxic media preparation (MNCC) Mouse Neuronal Cell Culture Complete Growth Media with 10% Fetal Bovine Serum (FBS) Celprogen M11003-02S For maintenance of MNCC, used for normoxic conditions; also referred to as regular/normal media Mouse Neuronal Cell Culture Extracellular Matrix – 96 Black Wall Well Plates (5/pk) Celprogen E1 1003-02-96BW For fluorometric-based assays (cf.

Techniques: Sparging, Cell Culture, Sterility, Cell Counting, Proliferation Assay, End Point Assay, Activity Assay, CyQUANT Assay, Transferring, Light Microscopy, Saline, LDH Cytotoxicity Assay, Membrane

Journal: International Journal of Molecular Sciences

Article Title: Time Does Matter: The Cellular Response to Resveratrol Varies Depending on the Exposure Duration

doi: 10.3390/ijms26125542

Figure Lengend Snippet: Assessment of the inhibitory concentration of resveratrol for human cardiomyocytes ( A ) and the influence of different incubation times at resveratrol concentration 5 µM on clonogenic survival of cardiomyocytes ( B ). Numbers of surviving clones are presented in relation to untreated controls (100%); statistically significant differences are marked with asterisks ( p < 0.05).

Article Snippet: Human cardiomyocytes were obtained from Celprogen (#1311001-09, Torrance, CA, USA) and cultured in flasks covered with fibronectin.

Techniques: Concentration Assay, Incubation, Clone Assay

Journal: International Journal of Molecular Sciences

Article Title: Time Does Matter: The Cellular Response to Resveratrol Varies Depending on the Exposure Duration

doi: 10.3390/ijms26125542

Figure Lengend Snippet: The influence of different incubation times with resveratrol on the mitochondrial metabolic activity of cardiomyocytes was measured using the XTT test ( A ). The relative activity is presented in relation to untreated controls (100%); statistically significant differences are marked with asterisks ( p < 0.05). Morphology of control (0 µM) and resveratrol-treated (5 µM) cardiomyocytes observed under light microscopy ( B ).

Article Snippet: Human cardiomyocytes were obtained from Celprogen (#1311001-09, Torrance, CA, USA) and cultured in flasks covered with fibronectin.

Techniques: Incubation, Activity Assay, Control, Light Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Time Does Matter: The Cellular Response to Resveratrol Varies Depending on the Exposure Duration

doi: 10.3390/ijms26125542

Figure Lengend Snippet: The influence of different incubation times with resveratrol on apoptosis of cardiomyocytes. Cells were stained with PI and anti-AnnexinV antibody, and analyzed by flow cytometry to estimate the number of apoptotic cells (PE and FITC-positive) ( A , B ). Activated caspase 3 was analyzed by Western blots using an antibody specific for the cleaved/activated form ( C ); lanes marked: 1—positive control (heat-induced apoptosis), 2—no resveratrol (control), 3—1 day, 4—1 week, 5—1 month of resveratrol treatment.

Article Snippet: Human cardiomyocytes were obtained from Celprogen (#1311001-09, Torrance, CA, USA) and cultured in flasks covered with fibronectin.

Techniques: Incubation, Staining, Flow Cytometry, Western Blot, Positive Control, Control

Journal: International Journal of Molecular Sciences

Article Title: Time Does Matter: The Cellular Response to Resveratrol Varies Depending on the Exposure Duration

doi: 10.3390/ijms26125542

Figure Lengend Snippet: The influence of different incubation times with resveratrol on cell cycle phase distribution of cardiomyocytes. Numbers of cells in different phases were counted by flow cytometry and are presented as histograms showing the distribution of cell populations in different cycle phases ( A ), and the G1:G2/M ratio ( B ); the differences between tested groups were not statistically significant.

Article Snippet: Human cardiomyocytes were obtained from Celprogen (#1311001-09, Torrance, CA, USA) and cultured in flasks covered with fibronectin.

Techniques: Incubation, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Time Does Matter: The Cellular Response to Resveratrol Varies Depending on the Exposure Duration

doi: 10.3390/ijms26125542

Figure Lengend Snippet: Changes in the levels of selected proteins in cardiomyocytes incubated for different times with resveratrol. Presented are representative Western blots and the relative quantification of analyzed proteins after normalization to β-actin (loading control) in relation to untreated controls (100%).

Article Snippet: Human cardiomyocytes were obtained from Celprogen (#1311001-09, Torrance, CA, USA) and cultured in flasks covered with fibronectin.

Techniques: Incubation, Western Blot, Quantitative Proteomics, Control

Journal: PLoS ONE

Article Title: The In Vitro Effect of Acidic-Pepsin on Nuclear Factor KappaB Activation and Its Related Oncogenic Effect on Normal Human Hypopharyngeal Cells

doi: 10.1371/journal.pone.0168269

Figure Lengend Snippet: Luciferase assay demonstrates the effect of pepsin in NF-κB transcriptional activity in (A) HHK and (B) HHPC. Acidic-pepsin (pH 4.0) does not induce NF-κB transcriptional activity in treated human normal hypopharyngeal cells. Columns represent NF-κB relative transcriptional activity (NF-κB responsive luciferase reporter/control luciferase reporter). 3kB-ConA-luc: NF-κB responsive luciferase reporter; ConA-luc: control luciferase reporter; 3kB-ConA-luc/ConA-luc: NF-κB responsive luciferase reporter/control luciferase reporter. CA: Acid-Control; PA: Acidic-Pepsin; CW: Weakly Acidic-Control; PW: Weakly Acidic-Pepsin; CN: Neutral-Control; PN: Neutral-Pepsin; PI: Inactivated-Pepsin.

Article Snippet: The HHPC were plated in non-coated flasks and were grown in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen Inc. CA, USA), at 37°C in humidified air and 5% CO 2 .

Techniques: Luciferase, Activity Assay